5075101: Method for diagnosis of fatty acid or lipid abnormalities

Inventors: Siguel; Edward N., Brookline, MA 02146-0001

Issued Dec. 24, 1991

Serial # 507659

ABSTRACT

A method for measuring more accurately Fatty Acids and Essential Fatty Acid (EFA) Deficiencies (EFAD). The invention includes a disease diagnostic method for the diagnosis of lipid and fatty acid biochemical abnormalities found through the analysis of tissues of a test subject, deriving indices of lipid and fatty acid biochemical status, and analytically comparing patterns or domains obtained from indices of a subject with similar indices derived from tissues of subjects with normal and abnormal biochemistry, for the purposes of diagnosing abnormalities. A specific mixture of nutrients (test mixture), prepared according to this invention, is given to a test subject to modify the tissue composition of said test subject in order to verify or modify a diagnosis, including mixtures of w3 and w6 fatty acids. Another specific mixture of mixture of nutrients (treatment mixture), prepared according to this invention, is given to a test subject to correct lipid and fatty acid abnormalities found in said test subject.

Part of Claim 1

What is claimed is:

1. A method of characterizing an abnormality of lipid or fatty acid biochemistry or metabolism in a human test subject by analyzing substances in a body tissue of said test subject and calculating indices derived from the quantity of each substance thus obtained, said method comprising the steps of:

(a) obtaining at least one tissue sample from a test subject;

(b) separating and quantifying at least two individual substances in said tissue sample(s) of said test subject to obtain the variables concentration and/or percent of each substance, including the calculation of percents of one substance as a percent of the total amount of substances within a specified group of similar substances;

(c) defining new variables as functions of the concentration and/or percents of said substances, including formulas that depend on the arithmetic operations of addition, subtraction, division and multiplication, wherein one of the variables is a concentration of one of the substances and another of the variables is a percent of a substance or ratio of concentrations of substances;

(d) calculating indices of the biochemical status of said test subject, and p1

(e) representing the relationships among two or more of said indices into a characteristic pattern, including a point in multidimensional space called the characteristic index, or equivalent representations in the form of tables.

OTHER REFERENCES:

Siguel, E. N. et al, Clinical Chemistry, 33:1869-1873 (1987).
Siguel, E. N., Nutritional Support Services, 8(9):24 (1988).
Holman, R. T., American J. Clinical Nutrition , 32:2390-2399 (1979).
Lundberg, Wo, Nutrition Reviews, 38(7):233-5 (1980).
Siguel, E. N., Nutrition and Cancer, 4(4):285-9 (1983).
Siguel, E. N., Arch. Pathol. Lab. Med., 110:792-7 (1986).

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